Isocorydine inhibits the proliferation of human endometrial carcinoma HEC-1B cells by downregulating the Ras/MEK/ERK signaling pathway

Objective: Isocorydine (ICD), an aporphine alkaloid, plays a role in anticancer activity that is still being evaluated. However, the exact function and mechanism of ICD endometrial carcinoma largely unknown. Methods: MTT, flow cytometry, western blot, immunofluorescence, RT-PCR ELISA were used this research. Results: In our study we showed inhibited HEC-1B cell proliferation at certain times concentrations. Simultaneously, induced cells apoptosis. Further investigation indicated reduced phosphorylation protein levels c-Raf (rapidly accelerated fibrosarcoma), MEK1/2 (MAPK/Erk kinase) ERK1/2 (extracellular regulated kinase). Moreover, immunofluorescence assay confirmed intracellular expression Ras then promoted FOXO3 (forkhead box O3) nuclear localization to furtherly increase mRNA level Bim p27Kip1. Additionally, enzyme-linked immunosorbent (ELISA) revealed production EGF (epidermal growth factor) PDGF (Platelet-Derived Growth Factor), which indirectly activation Ras/MEK/ERK signaling pathway ligand level. Conclusion: Taken together, these data suggest for first time inhibits induces apoptosis through suppressing signaling, may provide theoretical foundation application treatment human carcinoma.